![]() ![]() With a convenience sample of individual mosquitoes comprising 19 species from six genera in Saint Kitts ( n = 77) and Auburn ( n = 48), our AUCOS provided higher-quality sequence data than FCOS. ![]() Two molecular assays (AUCOS, FCOS) and morphological identification were carried out on mosquitoes collected from the field in Auburn, Alabama (USA) and on Saint Kitts. Sequence data in GenBank for 33 species from 10 genera of mosquitoes were used to develop our AUCOS primers. MethodsĪs there can be difficulties identifying mosquitoes using FCOS, we re-evaluated the FCOS primers and developed a new COI-based SYBR PCR (the Auburn COI system-AUCOS) to improve the molecular identification of mosquitoes. Problems with the morphological identification of mosquito species have led to the use of molecular identification techniques, in particular the Folmer cytochrome c oxidase subunit I ( COI) PCR system (FCOS), originally designed to identify a range of other invertebrates. Accurate identification of mosquito species is essential for the development and optimization of strategies to control mosquitoes and mosquito-borne diseases.
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